
T (+98) 23 352 20220
Email: international@du.ac.ir
Damghan University
University Blvd, Damghan, IR
Assistant Professor of Anatomical Sciences
AUTHOR KEYWORDS: Estradiol; Mice; Progesterone; Stem cells; Uterine
INDEX KEYWORDS: estradiol; messenger RNA; octamer transcription factor 4; progesterone; steroid hormone; transcription factor Sox2, animal experiment; animal model; animal tissue; Article; controlled study; gene sequence; immunofluorescence; menopause; mouse; nonhuman; ovariectomy; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; sampling; signal transduction; uterine tissue
PUBLISHER: Research and Clinical Center for Infertitlity
DOI: 10.1016/j.jep.2016.04.013
AUTHOR KEYWORDS: Co-culture; Colonization; Date palm pollen; Sertoli cell; Spermatogonial stem cell
INDEX KEYWORDS: cytokeratin; octamer transcription factor 4; Ddx4 protein, mouse; DEAD box protein; fizzy related protein; Fzr1 protein, mouse; Gfra1 protein, mouse; glial cell line derived neurotrophic factor receptor; octamer transcription factor 4; plant extract; Pou5f1 protein, mouse, animal cell; Article; coculture; colony formation; concentration (parameters); controlled study; date (fruit); gene expression; immunocytochemistry; in vitro study; male; mouse; newborn; nonhuman; pollen; polymerase chain reaction; protein expression; quantitative analysis; Sertoli cell; spermatogonial stem cell; spermatogonium; stem cell; animal; cell culture; cell proliferation; chemistry; cytology; drug effects; genetics; metabolism; Phoenix (plant); pollen; spermatogonium; stem cell, Animals; Animals, Newborn; Cdh1 Proteins; Cell Proliferation; Cells, Cultured; Coculture Techniques; DEAD-box RNA Helicases; Gene Expression; Glial Cell Line-Derived Neurotrophic Factor Receptors; Male; Mice; Octamer Transcription Factor-3; Phoeniceae; Plant Extracts; Pollen; Sertoli Cells; Spermatogonia; Stem Cells
PUBLISHER: Elsevier Ireland Ltd
AUTHOR KEYWORDS: Matrix metalloproteinases; Mouse; Pre antral follicles; Vitrification
INDEX KEYWORDS: gelatinase A; gelatinase B; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2, animal tissue; Article; female; gene expression; growth; metaphase; mouse; nonhuman; ovarian tissue; ovary follicle development; reverse transcription polymerase chain reaction; survival rate; vitrification
PUBLISHER: Research and Clinical Center for Infertitlity
DOI: 10.1080/14786419.2014.954115
AUTHOR KEYWORDS: date palm pollen; proliferation; spermatogonial stem cell; viability
INDEX KEYWORDS: fertility promoting agent; Phoenix dactylifera extract; plant extract; reactive oxygen metabolite; unclassified drug; plant extract, animal cell; apoptosis; aqueous solution; Article; cell count; cell proliferation; cell survival; cell suspension; cell viability; controlled study; date (fruit); drug cytotoxicity; in vitro study; male; mouse; newborn; nonhuman; Sertoli cell; spermatogonium; stem cell; survival rate; tissue engineering; tissue regeneration; animal; cell proliferation; chemistry; cytology; drug effects; Phoenix (plant); pollen; spermatogonium; testis, Animals; Cell Proliferation; Male; Mice; Phoeniceae; Plant Extracts; Pollen; Sertoli Cells; Spermatogonia; Testis
PUBLISHER: Taylor and Francis Ltd.
METHODS: Isolated pre-antral follicles (140-150 µm in diameter) were divided into vitrified-warmed and fresh groups. Each group was subjected to in vitro maturation with or without ALA for 12 days, followed by adding human chronic gonadotropin to induce ovulation. In vitro fertilization was performed to evaluate their developmental competence. In parallel, the amount of ROS and TAC were assessed after 0, 24, 48, 72, and 96 h of culture by 2',7'-dichlorofluorescin assay and ferric reducing/antioxidant power assay, respectively.
RESULTS: The respective rates of survival, antrum formation, and metaphase II oocytes were significantly higher in ALA-supplemented groups compared to the groups not treated with ALA. TAC and ROS levels were significantly decreased and increased, respectively during the culture period up to 96 h in the absence of ALA in both vitrified and non-vitrified samples. However, with pretreatment of ALA, TAC levels were increased significantly and remained constant up to 96 h in vitrified-warmed pre-antral follicles, while ROS levels completely returned to the level of starting point after 96 h of culture in the presence of ALA.
CONCLUSION: Pretreatment of ALA positively influences development of pre-antral follicles in vitrified and non-vitrified samples through increasing follicular TAC level and decreasing ROS levels.
INDEX KEYWORDS: antioxidant; reactive oxygen metabolite; thioctic acid, animal; cell culture; cell differentiation; drug effects; female; human; metabolism; mouse; oocyte; ovary follicle; oxidation reduction reaction; vitrification, Animals; Antioxidants; Cell Differentiation; Cells, Cultured; Female; Humans; Mice; Oocytes; Ovarian Follicle; Oxidation-Reduction; Reactive Oxygen Species; Thioctic Acid; Vitrification
AUTHOR KEYWORDS: Albumin; Glycogen; Hepatocytes; Liver genes; Mesenchymal stem cells
INDEX KEYWORDS: albumin; alpha fetoprotein; cytokeratin 18; cytokeratin 19; glycogen, animal tissue; article; cell density; chemical analysis; controlled study; electrophoresis; gene expression; immunocytochemistry; liver cell; liver function; male; mesenchymal stem cell; MTT assay; nonhuman; rat; reverse transcription polymerase chain reaction
AUTHOR KEYWORDS: Antioxidant enzymes; Matrix metalloproteinases; Mesenchymal stem cells; Replicative Cellular Senescence
INDEX KEYWORDS: glutathione peroxidase; matrix metalloproteinase; superoxide dismutase; antioxidant; glutathione peroxidase; superoxide dismutase, adipose derived stem cell; animal cell; antioxidant activity; article; cell culture; cell therapy; controlled study; enzymatic assay; hematopoietic stem cell; mesenchymal stem cell; nonhuman; protein expression; rat; replicative cellular senescence; zymography; hematopoietic stem cell
PUBLISHER: Semnan University of Medical Sciences
Materials and Methods: This was an experimental laboratory study. We assessed the effect of intravenous transplantation of adipose-derived stem cells (ADSCs) on hippocampal neurogenesis after inducing injury by 6-OHDA (memory disability model of PD).We performed bilateral injections of 6-OHDA into substantia nigra (SNc) of male Wistar rats. First group of the rats received bilateral injections of 6-OHDA (6 µg) dissolved in 2µl saline. Second group received saline injections instead of neurotoxin (sham group). In the third group we transplanted the 3rd passage of ADSC cells which had been assessed for CD90 immunostaining (1×106 in 500 μl medium), via tail vein. The 4th group included injured rats which received an injection of the fluid of the culture media (500 µl) through tail vein. After treatment, rats were sacrificed. The brains of the rats were removed, fixed with 4% paraformaldehyde, dehydrated, embedded in paraffin and cut into 10μm thick slices. We stained the sections with cresyl violet and determined the density of neurons in DG, CA1, CA3.
Result: Statistical analysis was performed by one-way ANOVA and Tukey test. P≤ 0 .05 was considered significant. Neuron density in DG, CA1, CA3 showed significant decrease in the injured and medium treated groups compared to the sham group (P<0.00). All so we found a significant increase in neuron density in these regions in the cell group in comparison to the medium treated and injured groups (p<0.000).
Conclusion: Intravenous injection of ADSCs protected hippocampal neurons from further damage in response to 6-OHDA.Therfore cell therapy can be a suitable method for the improvement of memory impairment in the patients with Parkinson's disease. © 2014, Kurdistan University of Medical Sciences. All rights reserved.
AUTHOR KEYWORDS: Adipose-derived stem cells; Hippocampus; Memory; Neurogenesis; Parkinson's disease
INDEX KEYWORDS: oxidopamine; Thy 1 antigen, adipose derived stem cell; animal model; animal tissue; Article; cell density; controlled study; dentate gyrus; hippocampal CA1 region; hippocampal CA3 region; hippocampus; immunohistochemistry; male; memory disorder; nerve injury; nervous system development; nonhuman; Parkinson disease; stem cell transplantation; substantia nigra
PUBLISHER: Kurdistan University of Medical Sciences
DOI: 10.1016/j.pbb.2014.05.020
INDEX KEYWORDS: Erratum; retracted article
PUBLISHER: Elsevier Inc.
AUTHOR KEYWORDS: 6-Hydroxydopamine; Carnosic acid; Dopamine; Hippocampus; Neuroprotective
INDEX KEYWORDS: carnosic acid; oxidopamine; Rosmarinus officinalis extract, animal experiment; animal model; animal tissue; article; brain injury; controlled study; hippocampal CA1 region; immunohistochemistry; male; memory disorder; nervous system development; neuroprotection; nonhuman; pyramidal nerve cell; rat
PUBLISHER: Semnan University of Medical Sciences
AUTHOR KEYWORDS: Alpha lipoic acid; Ovary; Preantral follicles; Vitrification
INDEX KEYWORDS: chorionic gonadotropin; thioctic acid, animal cell; animal tissue; Article; controlled study; embryo; embryo development; female; fertilization in vitro; in vitro study; intermethod comparison; male; mouse; nonhuman; oocyte maturation; ovary follicle; ovary follicle development; ovulation; preantral follicle; tissue culture; vitrification
PUBLISHER: Research and Clinical Center for Infertitlity
DOI: 10.6091/ibj.1258.2014
AUTHOR KEYWORDS: Alpha lipoic acid (ALA); Pre-antral follicle; Reactive oxygen species (ROS); Total antioxidant capacity (TAC); Vitrification
INDEX KEYWORDS: chorionic gonadotropin; dichlorodihydrofluorescein diacetate; reactive oxygen metabolite; thioctic acid, animal cell; antioxidant activity; antioxidant assay; article; blastocyte; cell culture; cell isolation; cell maturation; cell structure; cell survival; controlled study; enzyme assay; female; fertilization in vitro; in vitro study; metaphase; nonhuman; oocyte development; ovary follicle cell; ovulation; ovulation induction; oxidation; rat; vitrification
PUBLISHER: Pasteur Institute of Iran
AUTHOR KEYWORDS: ALA; cAMP-elevating agents; Cumulus cell; In vitro maturation; Mouse; Oocyte
INDEX KEYWORDS: cilostamide; cyclic AMP; forskolin; thioctic acid, animal cell; animal experiment; article; controlled study; cumulus cell; drug effect; embryo; embryo culture; female; germinal vesicle; in vitro oocyte maturation; male; mouse; nonhuman; oocyte maturation; spermatozoon; spermatozoon density
AUTHOR KEYWORDS: 6-Hydroxydopamine; Bone Marrow Stromal Cells; Cell Therapy; Deprenyl; Parkinson's Disease
INDEX KEYWORDS: apomorphine; broxuridine; oxidopamine; selegiline; tyrosine 3 monooxygenase, animal behavior; animal cell; animal experiment; animal model; animal tissue; article; bone marrow cell; cell differentiation; cell labeling; cell transplantation; cell ultrastructure; circling behavior; controlled study; culture medium; electron microscopy; experimental study; immunohistochemistry; incubation time; male; nonhuman; nucleotide sequence; Parkinson disease; rat; stroma cell
DOI: 10.1042/CBI20110618
AUTHOR KEYWORDS: ADSC; BMSC; Gene expression; Growth curve; Neurotrophic factors
INDEX KEYWORDS: biological marker; CD71 antigen; ciliary neurotrophic factor; glial cell line derived neurotrophic factor; nestin; neurotrophic factor; Thy 1 antigen, adipose tissue; animal cell; animal tissue; article; beta2M gene; bone marrow; cell assay; cell function; cell population; cell proliferation; cell viability; cellular distribution; CNTF gene; controlled study; DNA sequence; GDNF gene; gene; gene expression; immunocytochemistry; immunohistochemistry; in vitro study; male; mesenchymal stem cell; nestin gene; neurotrophic factor gene; NGF gene; nonhuman; NT3 gene; NT5 gene; nucleotide sequence; rat; reverse transcription polymerase chain reaction; structure analysis, Adipose Tissue; Animals; Bone Marrow Cells; Cell Proliferation; Cell Survival; Cells, Cultured; Gene Expression Regulation; Male; Mesenchymal Stromal Cells; Nerve Growth Factors; Polymerase Chain Reaction; Rats; Rats, Wistar; Stromal Cells
AUTHOR KEYWORDS: Bone marrow; Long-term culture; Mesenchymal stem cells; Neurotrophic factors; Spontaneous differentiation
INDEX KEYWORDS: cell nucleus receptor; nestin; neurotrophic factor; tyrosine 3 monooxygenase, analysis of variance; animal cell; article; cell culture; controlled study; hematopoietic stem cell; marker gene; mesenchymal stem cell; nonhuman; protein expression; rat; reverse transcription polymerase chain reaction; statistical analysis; stem cell
AUTHOR KEYWORDS: Conditioning; Ethanol; Hippocampus; Reconsolidation
INDEX KEYWORDS: alcohol; paraformaldehyde; pentobarbital; phosphate buffered saline, analysis of variance; animal cell; animal experiment; animal model; animal tissue; article; behavior; clinical examination; conditioning; controlled study; dentate gyrus; experimental study; fear; granule cell; hippocampal CA1 region; hippocampal CA3 region; histology; male; medical research; memory; nonhuman; perfusion; rat
DOI: 10.1016/j.pbb.2012.04.008
AUTHOR KEYWORDS: Contextual fear conditioning; Ethanol; Hippocampus; Reconsolidation
INDEX KEYWORDS: alcohol; paraformaldehyde; phosphate buffered saline, acute drug administration; animal cell; animal experiment; animal tissue; apoptosis; article; cell density; controlled study; dentate gyrus; drug effect; freezing; hippocampal CA1 region; hippocampal CA3 region; hippocampus; male; memory; nerve cell; nick end labeling; nonhuman; priority journal; rat; tissue fixation; tissue section, Animals; Apoptosis; Behavior, Animal; Ethanol; Hippocampus; Male; Memory; Neurons; Rats; Rats, Wistar, Animalia; Rattus; Rattus norvegicus
AUTHOR KEYWORDS: Cerebellum; Ethanol; Oxidative stress; Purkinje cell; Rat
INDEX KEYWORDS: alcohol; catalase; glutathione peroxidase; superoxide dismutase; thiobarbituric acid reactive substance, animal experiment; animal model; animal tissue; antioxidant activity; article; brain development; cerebellum vermis; controlled study; enzyme activity; histopathology; lipid peroxidation; male; microcephaly; neurotoxicity; newborn; nonhuman; oxidative stress; Purkinje cell; rat, Rattus
AUTHOR KEYWORDS: Bone marrow stromal cells; Cell therapy; Parkinson's disease; Stem cell
INDEX KEYWORDS: broxuridine; oxidopamine; tyrosine 3 monooxygenase, angiogenesis; animal cell; animal experiment; animal model; animal tissue; antibody labeling; article; bone marrow cell; cell transdifferentiation; cell transplantation; controlled study; corpus striatum; femur; immunohistochemistry; immunoreactivity; male; mouse; neurofilament; nonhuman; parkinsonism; phenotype; rotation; stroma cell
DOI: 10.1016/j.pbb.2011.07.010
AUTHOR KEYWORDS: 17β-estradiol; Ethanol; Lipid peroxidation; Oxidative stress; Purkinje cell; Rat pups
INDEX KEYWORDS: alcohol; catalase; estradiol; glutathione peroxidase; superoxide dismutase; thiobarbituric acid reactive substance, animal experiment; antioxidant activity; article; behavior change; biochemistry; brain development; cerebellum; cerebellum vermis; controlled study; female; histology; locomotion; male; neuroprotection; neurotoxicity; nonhuman; oxidative stress; perinatal period; priority journal; Purkinje cell; rat; rotarod test; task performance, Animals; Animals, Newborn; Cerebellum; Estradiol; Ethanol; Male; Motor Activity; Motor Skills Disorders; Neuroprotective Agents; Oxidative Stress; Rats; Rats, Wistar, Rattus
AUTHOR KEYWORDS: Bone marrow stromal cells; Neurotrophic factors; Selegiline
INDEX KEYWORDS: azacitidine; brain derived neurotrophic factor; cell marker; epidermal growth factor; glial cell line derived neurotrophic factor; glial fibrillary acidic protein; microtubule associated protein 1; nerve growth factor; neuron specific nuclear protein; neurotrophin 3; neurotrophin 4; selegiline; selenium; transferrin; vimentin, article; autograft; bone marrow cell; cell count; cell culture; cell differentiation; cell fate; cell transdifferentiation; cell viability; colony forming unit; controlled study; culture medium; dose response; gene expression; immunohistochemistry; in vitro study; incubation time; mesenchymal stem cell; molecular mechanics; nerve cell lesion; nerve cell plasticity; neurofilament; neurologic disease; nonhuman; stroma cell