
T (+98) 23 352 20220
Email: international@du.ac.ir
Damghan University
University Blvd, Damghan, IR
Assistant Professor of Anatomical Sciences
Materials and Methods: This was an experimental laboratory study. We assessed the effect of intravenous transplantation of adipose-derived stem cells (ADSCs) on hippocampal neurogenesis after inducing injury by 6-OHDA (memory disability model of PD).We performed bilateral injections of 6-OHDA into substantia nigra (SNc) of male Wistar rats. First group of the rats received bilateral injections of 6-OHDA (6 µg) dissolved in 2µl saline. Second group received saline injections instead of neurotoxin (sham group). In the third group we transplanted the 3rd passage of ADSC cells which had been assessed for CD90 immunostaining (1×106 in 500 μl medium), via tail vein. The 4th group included injured rats which received an injection of the fluid of the culture media (500 µl) through tail vein. After treatment, rats were sacrificed. The brains of the rats were removed, fixed with 4% paraformaldehyde, dehydrated, embedded in paraffin and cut into 10μm thick slices. We stained the sections with cresyl violet and determined the density of neurons in DG, CA1, CA3.
Result: Statistical analysis was performed by one-way ANOVA and Tukey test. P≤ 0 .05 was considered significant. Neuron density in DG, CA1, CA3 showed significant decrease in the injured and medium treated groups compared to the sham group (P<0.00). All so we found a significant increase in neuron density in these regions in the cell group in comparison to the medium treated and injured groups (p<0.000).
Conclusion: Intravenous injection of ADSCs protected hippocampal neurons from further damage in response to 6-OHDA.Therfore cell therapy can be a suitable method for the improvement of memory impairment in the patients with Parkinson's disease. © 2014, Kurdistan University of Medical Sciences. All rights reserved.
AUTHOR KEYWORDS: Adipose-derived stem cells; Hippocampus; Memory; Neurogenesis; Parkinson's disease
INDEX KEYWORDS: oxidopamine; Thy 1 antigen, adipose derived stem cell; animal model; animal tissue; Article; cell density; controlled study; dentate gyrus; hippocampal CA1 region; hippocampal CA3 region; hippocampus; immunohistochemistry; male; memory disorder; nerve injury; nervous system development; nonhuman; Parkinson disease; stem cell transplantation; substantia nigra
PUBLISHER: Kurdistan University of Medical Sciences
AUTHOR KEYWORDS: Antioxidant enzymes; Matrix metalloproteinases; Mesenchymal stem cells; Replicative Cellular Senescence
INDEX KEYWORDS: glutathione peroxidase; matrix metalloproteinase; superoxide dismutase; antioxidant; glutathione peroxidase; superoxide dismutase, adipose derived stem cell; animal cell; antioxidant activity; article; cell culture; cell therapy; controlled study; enzymatic assay; hematopoietic stem cell; mesenchymal stem cell; nonhuman; protein expression; rat; replicative cellular senescence; zymography; hematopoietic stem cell
PUBLISHER: Semnan University of Medical Sciences