
T (+98) 23 352 20220
Email: international@du.ac.ir
Damghan University
University Blvd, Damghan, IR
Associate Professor of Cell and Molecular Biology
Dr. Saeed Zavareh is currently associate professor in the department of Cell and Molecular Biology of Damghan University in Iran. He also holds the positions as consultant and researcher of the reproductive biology unit at the Institute of Biological Sciences in Damghan University. He received his Ph.D. degree in Theriogenology from Urmia University, and his Ph.D. thesis was partly done at University of Tarbiat Modares, Iran. His primary area of research focused on developing novel methods in fertility preservation including cryopreservation of ovarian tissue, oocyte and embryo. He also interested to study the role of stem cells in reproductive biology.
Theriogenology
Urmia University, Urmia, Iran
Veterinary Medicine
Urmia University, Urmia, Iran
DOI: 10.1016/j.msec.2018.09.005
AUTHOR KEYWORDS: Breast cancer; Magnetic nanoparticles; Poly L‑DOPA; Taxol®
INDEX KEYWORDS: Absorption spectroscopy; Amino acids; Atomic absorption spectrometry; Controlled drug delivery; Diseases; High performance liquid chromatography; Iron oxides; Magnetic fields; Magnetite; Mammals; Nanocomposites; Nanomagnetics; Nanoparticles; Tumors, Anti-tumor efficacy; Breast Cancer; Drug delivery system; Flame atomic absorption spectrometry; Magnetic nano-particles; Magnetic nanocomposites; Tumor tissues; Well-dispersed, Targeted drug delivery, indole derivative; magnetite nanoparticle; nanocomposite; paclitaxel; polydopamine; polymer, animal; Bagg albino mouse; chemistry; drug delivery system; experimental mammary neoplasm; female; metabolism; mouse; pathology; procedures, Animals; Drug Delivery Systems; Female; Indoles; Magnetite Nanoparticles; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Nanocomposites; Paclitaxel; Polymers
DOI: 10.29252/ijrm.16.2.69
AUTHOR KEYWORDS: Beta catenin; Estrous cycle; Mice; Wnt signaling pathway
INDEX KEYWORDS: beta catenin; Wnt protein, adult; animal experiment; animal tissue; Article; controlled study; estrus cycle; female; gene expression; histology; mouse; nonhuman; ovary tissue; reverse transcription polymerase chain reaction; signal transduction; uterus horn; vagina cytology
DOI: 10.1016/j.reactfunctpolym.2017.10.025
AUTHOR KEYWORDS: Doxorubicin; Epitope; HER2; Imprinted polymer; Ovarian cancer
INDEX KEYWORDS: Bioelectric phenomena; Biosynthesis; Conformations; Diseases; Mammals; Polymers; Proteins; Silica; Synthesis (chemical); Targeted drug delivery; Tissue; Tumors, Conformational epitopes; Doxorubicin; HER2; Imprinted polymers; Molecular imprinted polymers; Molecularly Imprinted Polymer; Ovarian cancers; Three dimensional space, Epitopes
DOI: 10.1111/jog.13173
AUTHOR KEYWORDS: antioxidant enzyme; coenzyme Q10; ovary; vitrification
INDEX KEYWORDS: catalase; glutathione peroxidase; malonaldehyde; superoxide dismutase; ubidecarenone; antioxidant; catalase; glutathione peroxidase; malonaldehyde; superoxide dismutase; ubidecarenone; ubiquinone, animal cell; animal tissue; Article; controlled study; culture medium; enzyme activity; female; lipid peroxidation; metabolic parameters; mouse; nonhuman; ovary follicle; ovary tissue; total antioxidant capacity; vitrification; analogs and derivatives; animal; drug effects; enzymology; metabolism; ovary follicle, Animals; Antioxidants; Catalase; Female; Glutathione Peroxidase; Malondialdehyde; Mice; Ovarian Follicle; Superoxide Dismutase; Ubiquinone; Vitrification
DOI: 10.1080/19396368.2016.1235236
AUTHOR KEYWORDS: Coenzyme Q10; lipid peroxidation; preantral follicles; vitrification
INDEX KEYWORDS: antioxidant; catalase; glutathione peroxidase; lipid peroxide; malonaldehyde; superoxide dismutase; ubidecarenone; antioxidant; catalase; glutathione peroxidase; malonaldehyde; superoxide dismutase; ubidecarenone; ubiquinone, animal cell; antioxidant activity; Article; cell culture; cell maturation; controlled study; enzyme activity; in vitro study; lipid peroxidation; mouse; nonhuman; ovary; ovary follicle; ovary follicle cell; ovary follicle development; ovary follicle fluid; priority journal; total antioxidant capacity; vitrification; warming; analogs and derivatives; animal; drug effects; enzymology; female; metabolism; ovary follicle, Animals; Antioxidants; Catalase; Female; Glutathione Peroxidase; Lipid Peroxidation; Malondialdehyde; Mice; Ovarian Follicle; Superoxide Dismutase; Ubiquinone; Vitrification
DOI: 10.1007/s00280-016-3169-5
AUTHOR KEYWORDS: Breast adenocarcinoma; Doxorubicin; Imprinted polydopamine; Magnetic nanoparticle
INDEX KEYWORDS: dopamine derivative; doxorubicin; magnetic nanoparticle; polydopamine; unclassified drug; antineoplastic antibiotic; biocompatible coated material; delayed release formulation; doxorubicin; indole derivative; magnetite nanoparticle; polydopamine; polymer, animal experiment; animal model; animal tissue; Article; atomic absorption spectrometry; breast adenocarcinoma; controlled study; drug delivery system; drug distribution; drug release; drug synthesis; female; growth inhibition; high performance liquid chromatography; histopathology; in vitro study; in vivo study; infrared spectroscopy; magnetic field; mitosis index; mouse; nonhuman; priority journal; scanning electron microscopy; surface property; survival rate; tumor growth; tumor volume; adenocarcinoma; animal; Bagg albino mouse; chemistry; delayed release formulation; electromagnetism; Mammary Neoplasms, Animal; metabolism; particle size; survival analysis; synthesis; tissue distribution; treatment outcome, Adenocarcinoma; Animals; Antibiotics, Antineoplastic; Coated Materials, Biocompatible; Delayed-Action Preparations; Doxorubicin; Drug Delivery Systems; Electromagnetic Fields; Indoles; Magnetite Nanoparticles; Mammary Neoplasms, Animal; Mice; Mice, Inbred BALB C; Particle Size; Polymers; Spectrophotometry, Atomic; Spectroscopy, Fourier Transform Infrared; Survival Analysis; Tissue Distribution; Treatment Outcome
AUTHOR KEYWORDS: Estradiol; Mice; Progesterone; Stem cells; Uterine
INDEX KEYWORDS: estradiol; messenger RNA; octamer transcription factor 4; progesterone; steroid hormone; transcription factor Sox2, animal experiment; animal model; animal tissue; Article; controlled study; gene sequence; immunofluorescence; menopause; mouse; nonhuman; ovariectomy; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; sampling; signal transduction; uterine tissue
AUTHOR KEYWORDS: Ovarian follicle; Ovary; Vitrification
INDEX KEYWORDS: animal tissue; Article; cell survival; controlled study; developmental stage; mouse; nonhuman; oocyte; ovary follicle; vitrification
AUTHOR KEYWORDS: Matrix metalloproteinases; Mouse; Pre antral follicles; Vitrification
INDEX KEYWORDS: gelatinase A; gelatinase B; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2, animal tissue; Article; female; gene expression; growth; metaphase; mouse; nonhuman; ovarian tissue; ovary follicle development; reverse transcription polymerase chain reaction; survival rate; vitrification
DOI: 10.1016/j.ijpharm.2015.11.040
AUTHOR KEYWORDS: 5-Fluorouracil; Imprinted polymer; Magnetic nanoparticles; Polydopamine
INDEX KEYWORDS: fluorouracil; iron; magnetic nanoparticle; molecularly imprinted polymer; polymer; antineoplastic antimetabolite; fluorouracil; indole derivative; magnetite nanoparticle; polydopamine, animal experiment; animal model; animal tissue; Article; atomic absorption spectrometry; breast adenocarcinoma; cancer inhibition; controlled drug release; controlled study; drug distribution; drug efficacy; high performance liquid chromatography; histopathology; infrared spectroscopy; kidney parenchyma; liver; magnetic field; mouse; nonhuman; particle size; priority journal; survival rate; animal; Bagg albino mouse; Breast Neoplasms; chemistry; disease model; drug delivery system; drug release; female; procedures; survival analysis; tissue distribution; tumor cell line; ultrastructure, Animals; Antimetabolites, Antineoplastic; Breast Neoplasms; Cell Line, Tumor; Disease Models, Animal; Drug Delivery Systems; Drug Liberation; Female; Fluorouracil; Indoles; Magnetic Fields; Magnetite Nanoparticles; Mice; Mice, Inbred BALB C; Particle Size; Polymers; Survival Analysis; Tissue Distribution
AUTHOR KEYWORDS: Alpha lipoic acid; Oocytes maturation; Oxidative statuse
INDEX KEYWORDS: cilostamide; forskolin; reactive oxygen metabolite; thioctic acid, animal cell; antioxidant assay; Article; controlled study; female; in vitro oocyte maturation; mouse; nonhuman; oocyte maturation
METHODS: Isolated pre-antral follicles (140-150 µm in diameter) were divided into vitrified-warmed and fresh groups. Each group was subjected to in vitro maturation with or without ALA for 12 days, followed by adding human chronic gonadotropin to induce ovulation. In vitro fertilization was performed to evaluate their developmental competence. In parallel, the amount of ROS and TAC were assessed after 0, 24, 48, 72, and 96 h of culture by 2',7'-dichlorofluorescin assay and ferric reducing/antioxidant power assay, respectively.
RESULTS: The respective rates of survival, antrum formation, and metaphase II oocytes were significantly higher in ALA-supplemented groups compared to the groups not treated with ALA. TAC and ROS levels were significantly decreased and increased, respectively during the culture period up to 96 h in the absence of ALA in both vitrified and non-vitrified samples. However, with pretreatment of ALA, TAC levels were increased significantly and remained constant up to 96 h in vitrified-warmed pre-antral follicles, while ROS levels completely returned to the level of starting point after 96 h of culture in the presence of ALA.
CONCLUSION: Pretreatment of ALA positively influences development of pre-antral follicles in vitrified and non-vitrified samples through increasing follicular TAC level and decreasing ROS levels.
INDEX KEYWORDS: antioxidant; reactive oxygen metabolite; thioctic acid, animal; cell culture; cell differentiation; drug effects; female; human; metabolism; mouse; oocyte; ovary follicle; oxidation reduction reaction; vitrification, Animals; Antioxidants; Cell Differentiation; Cells, Cultured; Female; Humans; Mice; Oocytes; Ovarian Follicle; Oxidation-Reduction; Reactive Oxygen Species; Thioctic Acid; Vitrification
DOI: 10.1111/jog.12394
AUTHOR KEYWORDS: alpha lipoic acid; ovarian tissue; oxidative status; vitrification
INDEX KEYWORDS: reactive oxygen metabolite; thioctic acid; antioxidant; reactive oxygen metabolite; thioctic acid, animal cell; article; blastocyst; cell survival; comparative study; controlled study; embryo; embryo cleavage; embryo development; female; fertilization in vitro; mouse; nonhuman; oocyte; ovary follicle; oxidative stress; thawing; vitrification; vitrified warmed ovarian tissue; vitrified warmed preantral follicle; animal; cryopreservation; drug effects; fertilization; metabolism; ovary follicle, Animals; Antioxidants; Cryopreservation; Embryonic Development; Female; Fertilization; Mice; Ovarian Follicle; Reactive Oxygen Species; Thioctic Acid; Vitrification
DOI: 10.6091/ibj.1258.2014
AUTHOR KEYWORDS: Alpha lipoic acid (ALA); Pre-antral follicle; Reactive oxygen species (ROS); Total antioxidant capacity (TAC); Vitrification
INDEX KEYWORDS: chorionic gonadotropin; dichlorodihydrofluorescein diacetate; reactive oxygen metabolite; thioctic acid, animal cell; antioxidant activity; antioxidant assay; article; blastocyte; cell culture; cell isolation; cell maturation; cell structure; cell survival; controlled study; enzyme assay; female; fertilization in vitro; in vitro study; metaphase; nonhuman; oocyte development; ovary follicle cell; ovulation; ovulation induction; oxidation; rat; vitrification
AUTHOR KEYWORDS: Alpha lipoic acid; Ovary; Preantral follicles; Vitrification
INDEX KEYWORDS: chorionic gonadotropin; thioctic acid, animal cell; animal tissue; Article; controlled study; embryo; embryo development; female; fertilization in vitro; in vitro study; intermethod comparison; male; mouse; nonhuman; oocyte maturation; ovary follicle; ovary follicle development; ovulation; preantral follicle; tissue culture; vitrification
AUTHOR KEYWORDS: ALA; cAMP-elevating agents; Cumulus cell; In vitro maturation; Mouse; Oocyte
INDEX KEYWORDS: cilostamide; cyclic AMP; forskolin; thioctic acid, animal cell; animal experiment; article; controlled study; cumulus cell; drug effect; embryo; embryo culture; female; germinal vesicle; in vitro oocyte maturation; male; mouse; nonhuman; oocyte maturation; spermatozoon; spermatozoon density
DOI: 10.1155/2013/489032
INDEX KEYWORDS: adenosine triphosphate; calcium; flou 4 am; fluorescent dye; JC 1; rhodamine 123; unclassified drug; adenosine triphosphate; adenosine triphosphate, animal cell; animal experiment; animal tissue; article; blastocyst; calcium cell level; cell culture; cell function; cell isolation; cell maturation; cellular distribution; confocal laser microscopy; controlled study; cryopreservation; developmental stage; embryo; embryo development; female; fertilization; fertilization in vitro; germinal vesicle; in vitro study; in vivo study; metaphase; mitochondrial membrane potential; mitochondrion; mouse; nonhuman; oocyte; ovary; ovary tissue; staining; superovulation; vitrification; animal; cell survival; cytology; metabolism; mitochondrion; physiology; vitrification; metabolism; oocyte; ovary, Adenosine Triphosphate; Animals; Blastocyst; Cell Survival; Cryopreservation; Female; Fertilization; Fertilization in Vitro; Membrane Potential, Mitochondrial; Mice; Mitochondria; Oocytes; Ovary; Vitrification, Adenosine Triphosphate; Animals; Blastocyst; Cell Survival; Cryopreservation; Female; Fertilization; Fertilization in Vitro; Membrane Potential, Mitochondrial; Mice; Mitochondria; Oocytes; Ovary; Vitrification
AUTHOR KEYWORDS: Embryo; In vitro maturation; Oocyte; Progesterone
INDEX KEYWORDS: cholesterol; granulocyte macrophage colony stimulating factor; luteinizing hormone; pregnenolone; progesterone; steroid hormone, blastocyst; culture medium; embryo development; fertilization; gene expression; hormone substitution; human; nonhuman; oocyte maturation; ovary function; ovary polycystic disease; review; Western blotting
DOI: 10.1016/j.pbb.2012.04.008
AUTHOR KEYWORDS: Contextual fear conditioning; Ethanol; Hippocampus; Reconsolidation
INDEX KEYWORDS: alcohol; paraformaldehyde; phosphate buffered saline, acute drug administration; animal cell; animal experiment; animal tissue; apoptosis; article; cell density; controlled study; dentate gyrus; drug effect; freezing; hippocampal CA1 region; hippocampal CA3 region; hippocampus; male; memory; nerve cell; nick end labeling; nonhuman; priority journal; rat; tissue fixation; tissue section, Animals; Apoptosis; Behavior, Animal; Ethanol; Hippocampus; Male; Memory; Neurons; Rats; Rats, Wistar, Animalia; Rattus; Rattus norvegicus
DOI: 10.1007/s10815-011-9706-6
AUTHOR KEYWORDS: Alpha-lipoic acid; Preantral follicles; Reactive oxygen species; Total antioxidant capacity
INDEX KEYWORDS: antioxidant; reactive oxygen metabolite; thioctic acid, animal experiment; article; competence; controlled study; embryo development; female; fertilization in vitro; immaturity; mouse; nonhuman; oocyte maturation; ovary follicle development; priority journal; spectrofluorometry; survival rate, Animals; Cell Culture Techniques; Female; Fetus; Mice; Oocytes; Ovarian Follicle; Reactive Oxygen Species; Thioctic Acid
DOI: 10.1358/mf.2010.32.7.1487085
INDEX KEYWORDS: calcium; cannabis; cholesterol; estradiol; fatty acid; high density lipoprotein cholesterol; lipid; lipoprotein; calcium; estradiol; lipid; vegetable oil, article; behavior change; calcium blood level; cholesterol blood level; drug effect; estradiol blood level; forced swimming test; menopause; ovariectomy; plant seed; sham procedure; traditional medicine; animal; animal behavior; animal model; blood; female; human; menopause; rat; reproduction; Wistar rat, Animals; Behavior, Animal; Calcium; Cannabis; Estradiol; Female; Humans; Lipids; Menopause; Models, Animal; Ovariectomy; Plant Oils; Rats; Rats, Wistar; Reproductive Physiological Phenomena; Seeds